Characterisation of N-terminal chromogranin A and chromogranin B in mammals by region-specific radioimmunoassays and chromatographic separation methods
نویسندگان
چکیده
منابع مشابه
Library of sequence-specific radioimmunoassays for human chromogranin A.
BACKGROUND Human chromogranin A (CgA) is an acidic protein widely expressed in neuroendocrine tissue and tumors. The extensive tissue- and tumor-specific cleavages of CgA at basic cleavage sites produce multiple peptides. METHODS We have developed a library of RIAs specific for different epitopes, including the NH2 and COOH termini and three sequences adjacent to dibasic sites in the remainin...
متن کاملInteraction of chromogranin B and the near N-terminal region of chromogranin B with an intraluminal loop peptide of the inositol 1,4, 5-trisphosphate receptor.
Given the interaction of the inositol 1,4,5-trisphosphate receptor (IP(3)R) with chromogranins A (CGA) and B (CGB), two major Ca(2+) storage proteins of secretory granules that have been shown to be IP(3)-sensitive intracellular Ca(2+) store of neuroendocrine cells, we have investigated the potential interaction of the intraluminal loop regions of the IP(3)R with both intact CGB and the conserv...
متن کاملEffects of pH and Ca2+ on heterodimer and heterotetramer formation by chromogranin A and chromogranin B.
The two major proteins of the secretory vesicles of neuroendocrine cells, chromogranin A (CGA) and chromogranin B (CGB), have been shown to undergo pH- and Ca2+-dependent conformational changes and aggregation and have been suggested to play essential roles during secretory vesicle biogenesis in the trans-Golgi network. CGA has been shown to exist primarily in a tetrameric state at pH 5.5 and p...
متن کامل[Chromogranin A and neuroendocrine tumors].
Chromogranin A (CgA) is the most abundant granin in gastroenteropancreatic neuroendocrine tumors (GEP-NETs). As a tumor marker is moderately sensitive and nonspecific. Despite the limitations of testing methods, which require careful interpretation, especially in the case of gastrinomas, patients treated with somatostatin analogues, and poorly differentiated tumors, it is the best tumor marker ...
متن کاملChromogranin A and Neuroendocrine Tumors
A neuroendocrine tumor is suspected when classical clinical symptoms occur but the large majority of NETs does not show any specific symptomatology (Oberg et al., 1999). Accordingly, the biochemical diagnosis is of great value, with the validation of radioimmunoenzymatic assays for various circulating peptide hormones in the last decade, clinical awareness and ability to diagnose NET is increas...
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ژورنال
عنوان ژورنال: Journal of Endocrinology
سال: 2000
ISSN: 0022-0795,1479-6805
DOI: 10.1677/joe.0.1650703